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Beacon Designer Developer(s) Premier Biosoft Stable release 7.9 / 17 June 2010 Operating system Windows, Macintosh Type Bioinformatics License commercial Website http://www.premierbiosoft.com Beacon Designer designs highly specific an efficient primers and probes for real time PCR (polymerase chain reaction) assays. It is compatible to work on Windows as well as on Mac. The software currently supports the following real time PCR chemistries for efficient primer and probe design. 1. SYBR Green 2. TaqMan 3. Molecular beacon 4. HRMA Primers 5. Scorpions 6. FRET 7. LNA (Locked nucleic acid) 8. NASBA Beacon Designer program has an in-built support for homology avoidance using the standard web BLAST service or the set-up of a desktop BLAST feature for identification of regions that are significantly cross homologous with the target gene sequence. The software designs real time pcr primers and probes for complex differential gene expression and allele discrimination assays. Avoidance of the cross homology renders specificity to the primers in question and they are highly targeted and only amplify the intend sequence and no other targets. Beacon Designer has been used in workshops and conferences related to real time PCR study as a reference software. The author company, Premier Biosoft has also launched a simple version for evaluation of oligos for real time PCR primers and probes (support limited to TaqMan and SYBR Green primers) by the name Beacon designer free version. External links Beacon Designer Homepage Beacon Designer Free Edition High Resolution Melt